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TLS Online TPP Program
#Question id: 13058
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
TLS Online TPP Program
#Question id: 15141
#SCPH05 I Biotechnology
An alignment of two protein sequences is given below:
Sequence A A G A A C D E V I G
Sequence B A G E Y C D A V I G
The similarity score (%) for the above alignment will be:
TLS Online TPP Program
#Question id: 19130
#SCPH28 | Zoology
The biological component is suitably immobilized on to the transducer by which ways.
1. Enzymes are immobilized by glutaraldehyde
2. Porous sheet like lens tissue paper or nylon net fabric are used
3. Transducer surface may not be covalently linked to this cross-linked siloxane
Which of the statements is incorrect?
TLS Online TPP Program
#Question id: 15271
#SCPH01 Biochemistry
During RNA extraction, the homogenate is first treated with
TLS Online TPP Program
#Question id: 10600
#SCPH28 | Zoology
Which of the following is not feature of Type II survivorship curve?