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TLS Online TPP Program
#Question id: 12929
#SCPH01 Biochemistry
Temperature at which the kinetic energy of the molecules lowers to reduce the chances of base paired sticky ends parting before they have been stabilized.
TLS Online TPP Program
#Question id: 306
#SCPH28 | Zoology
The force responsible for dissolution of ionic compound in water is_________
TLS Online TPP Program
#Question id: 18074
#I Life Science/ Life Sciences Group – I-V
Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use
TLS Online TPP Program
#Question id: 12926
#SCPH05 I Biotechnology
PCR is undertaken with a fluorescence reporter / quencher pair called______
TLS Online TPP Program
#Question id: 13088
#SCPH01 Biochemistry
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 1’s plasmid is;