A 40-year-old woman visits the clinic complaining of fatigue. She had recently been treated for an infection. Her laboratory values are RBC 1.8 × 106/μl, Hb 5.2 g/dL, hematocrit (Hct) 15, WBC 7.6 × 103/μl, platelet count 320,000/μl, mean corpuscular volume (MCV) 92 fL, and reticulocyte count 24%. What is the most likely explanation for this presentation?

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#Question id: 13089

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

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#Question id: 23253

#SCPH01 Biochemistry

FISH is an in vitro assay that uses fluorescent reporter attached to a _______ that hybridizes to chromosomes.

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#Question id: 19199

#SCPH28 | Zoology

Example of calorimetric biosensor could be

1. Hydrogen peroxide and Catalase 

2.  Glucose oxidase

3. HCG and antibody 

4. Peptides and trypsin

Which among the following is the example?

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#Question id: 14368

#SCPH05 I Biotechnology

10 kg of a pure enzyme (Mr 30 000) catalyses the conversion of 1.5 g of a substrate (Mr 44) to products in 5 minutes. Calculate turnover number (sec^-1)  ....10^5

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#Question id: 10774

#SCPH05 I Biotechnology

Following are a few statements regarding the structure of terpenes:

a) Isopentenyl diphosphate and Dimethyallyl diphosphate are combinely called monoterpene, respectively

b) Isopentenyl diphosphate and Farnesyl diphosphate are Sesquiterpenes and Tetraterpenes

c) Monoterpenes, Sesquiterpenes and Diterpenes have 10, 20  and 30 carbons, respectively

d) Grenyl grenyl diphosphate usually have 20 carbons compound by multiplying with  2X is known as testraterpenes

Which combination of the above statements is correct?