TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 14820
#SCPH05 I Biotechnology
The smallest organism is probably determined by
TLS Online TPP Program
#Question id: 4322
#SCPH01 Biochemistry
If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp operon, then
TLS Online TPP Program
#Question id: 9207
#SCPH28 | Zoology
Loss of biodiversity matters not only with regard to mammals or other vertebrates, but also microbes. Why are microbes worthy of discovery and protection from extinction?
TLS Online TPP Program
#Question id: 13092
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?
TLS Online TPP Program
#Question id: 16027
#SCPH05 I Biotechnology
An established cell line can be called where it has been subcultured at least