Circus movements in the ventricle can lead to ventricular fibrillation. Which of the following conditions in the ventricular muscle will increase the tendency for circus movements?

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#Question id: 1296

#SCPH28 | Zoology

People with a defect in the synthesis of the integrin β2 subunit exhibit reduced:

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#Question id: 5753

#SCPH06 I Botany

A bacterial histidine mutant was plated on minimal medium and a single colony grew. You decide to sequence the histidine biosynthetic gene of the revertant and discover that the original mutation is still present.  This colony must have been able grow due to a

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#Question id: 27770

#Research Methodology

________research is based on the measurement of quantity or amount.

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#Question id: 16137

#SCPH01 Biochemistry

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)

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#Question id: 13089

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;