TLS Online TPP Program

#Question id: 11455


Statocysts contain cells that are ________.

#I Life Science/ Life Sciences Group – I-V
  1. mechanoreceptors used to detect orientation relative to gravity
  2. chemoreceptors used in selecting migration routes
  3. photoreceptors used in setting biological rhythms
  4. thermoreceptors used in prey detection
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TLS Online TPP Program

#Question id: 10274

#SCPH05 I Biotechnology

In plastids, a partial glycolysis occurs that produces metabolites for plastidial biosynthetic reactions, which of the following is not synthesized in the plastid?

TLS Online TPP Program

#Question id: 7306

#I Life Science/ Life Sciences Group – I-V

A plant scientist was hired by a greenhouse operator to devise a way to force iris plants to bloom in the short days of winter. Iris normally blooms as a long-day (short-night) plant. Which of the following has the best chance of creating iris blooms in winter?

TLS Online TPP Program

#Question id: 16128

#SCPH06 I Botany

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS. 
Next, you decide to evaluate the promoter for the GLN1 gene. To do this you first fuse the promoter region to the LacZ coding sequence and then place this hybrid gene on an appropriate yeast plasmid. You find that cells carrying the hybrid gene express activity under the same conditions that GS is expressed in wild type cells, meaning that the promoter region you have selected contains all of the necessary cis-acting sequences for normal regulation. The figure below shows the effect of different 50 bp deletions in the promoter region on the amount of ß-galactosidase activity expressed by the reporter gene. how would you expect a gln2– gln3– double mutant to behave?



TLS Online TPP Program

#Question id: 13087

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

TLS Online TPP Program

#Question id: 1279

#SCPH28 | Zoology

A tight junction is made up of which of the following proteins?

a. tricellulin                                                     b. occludin

c. claudin                                                         d. connexins