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#Question id: 4161
#SCPH06 I Botany
The link between an activated amino acid and tRNA is an __ bond between the __ of the amino acid and a hydroxyl group of the tRNA.
TLS Online TPP Program
#Question id: 13098
#SCPH05 I Biotechnology
You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
How many of the 6 potential open reading frames are actually open in this sequence shown above?
TLS Online TPP Program
#Question id: 13089
#SCPH01 Biochemistry
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 2’s plasmid is;
TLS Online TPP Program
#Question id: 9177
#SCPH28 | Zoology
Which of the following statements regarding turnover in a lake is correct?
TLS Online TPP Program
#Question id: 3636
#SCPH12 I Genetics
A man with type A blood group marries a woman with type A blood group. Their first child has type O blood. What is the genotype of the Man, proportion of his gametes are expected to contain the IA allele, and probability that their next child will have type A blood respectively: