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TLS Online TPP Program
#Question id: 13064
#SCPH05 I Biotechnology
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following techniques doesn’t involve use of a secondary antibody?
TLS Online TPP Program
#Question id: 3534
#SCPH01 Biochemistry
Start with a collection of mutants obtained using selections or screens and then characterize their wild functions at the molecular level called ----A------, alternatively, start with a set of specific DNA sequences, mutate them, and observe the phenotypes called --B-----.
TLS Online TPP Program
#Question id: 5616
#SCPH01 Biochemistry
In the sperm the elevated Ca2+ level in a relatively basic cytoplasm triggers
TLS Online TPP Program
#Question id: 14120
#SCPH01 Biochemistry
Which of the following is not a variant of BLAST?
TLS Online TPP Program
#Question id: 14663
#SCPH05 I Biotechnology
Identify the file format given below:
