In mevalonic acid pathway, three molecules of acetyl-CoA are joined together stepwise to form mevalonic acid, which intermediate steps it will perform to yield isopentenyl diphosphate (IPP2)?

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#Question id: 23163

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Out of the given below alexa dyes which one will emit in red light range?

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#Question id: 23164

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Out of the given below alexa dyes which one will emit in Blue light range?

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#Question id: 23165

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For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

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#Question id: 23166

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To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

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#Question id: 23167

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A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

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#Question id: 23168

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What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?