Match the items in Group 1 with an appropriate description in Group 2 

Group I                                      Group 2 

P. GOR method                           1. Protein structure visualization 

Q. Genome sequencing           2. Sanger method

R. ESTs                                         3. Protein secondary structure prediction

S. RasMol                                    4. gene expressed as cDNA

TLS Online TPP Program

#Question id: 14158

#SCPH05 I Biotechnology

BLAST uses a _______ to find matching words, whereas FASTA identifies identical matching words using the _____

TLS Online TPP Program

#Question id: 3690

#SCPH28 | Zoology

The DNA polymerase is a template-directed enzyme that synthesizes new complementary strand from a parent strand but it requires the existing short nucleotide sequence for its elongation. Which of the following enzyme is required for the synthesis of this primer?

TLS Online TPP Program

#Question id: 13058

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 441

#SCPH05 I Biotechnology

ATP is classified as an energy-rich compound because

TLS Online TPP Program

#Question id: 16032

#SCPH05 I Biotechnology

The major problem associated with the isolation of free cells and cell aggregates from organs is that of