in ab initio approach, generally when a base pairing is formed, the energy of the molecule is____ because of attractive interactions between the two strands.

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#Question id: 11149

#I Life Science/ Life Sciences Group – I-V

The Nernst equation specifies the equilibrium potential for a particular ion. This equilibrium potential is a function of ________.

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#Question id: 28020

#Research Methodology

Which of the following is the first step in starting the research process?

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#Question id: 659

#SCPH28 | Zoology

What does it mean to say a protein is oligomeric?

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#Question id: 13054

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 16477

#SCPH06 I Botany

Individual cell lineages are first isolated from an organ they are then grown separately to high density in a three- dimensional matrix. This  type of culture is called