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TLS Online TPP Program
#Question id: 13058
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
TLS Online TPP Program
#Question id: 4946
#SCPH28 | Zoology
Although absolute distinctions between the ʺmost evolvedʺ protobiont and the first living cell are unclear, biologists generally agree that one major difference is that the typical protobiont could not
TLS Online TPP Program
#Question id: 19110
#SCPH12 I Genetics
If the frequency of PKU among newborns is approximately 1/10,000 (when the parents are unrelated). What is the risk of a child with PKU if the parents are siblings?
TLS Online TPP Program
#Question id: 4344
#SCPH28 | Zoology
How does the transcription of structural genes in an inducible operon occur?
TLS Online TPP Program
#Question id: 14279
#SCPH05 I Biotechnology
Growth yield of mDicrobes is defined as