Nearly all peptide bonds are in the trans configuration because

TLS Online TPP Program

#Question id: 13073

#SCPH01 Biochemistry

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;

TLS Online TPP Program

#Question id: 11455

#I Life Science/ Life Sciences Group – I-V

Statocysts contain cells that are ________.

TLS Online TPP Program

#Question id: 17919

#SCPH01 Biochemistry

Which substitution matrices should you prefer to find distantly related orthologs through BLAST search?

TLS Online TPP Program

#Question id: 361

#SCPH06 I Botany

Calculate the percentage of histidine that has its imidazole side chain protonated at pH 7.3. The pKa values for histidine are pK1 = 1.8, pK2 (imidazole) = 6.0, and pK3 = 9.2.

TLS Online TPP Program

#Question id: 13054

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?