Which of the following is not known to be involved in the process of assisted folding of proteins?

TLS Online TPP Program

#Question id: 13089

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

TLS Online TPP Program

#Question id: 3652

#SCPH06 I Botany

Alkylating agents are electrophilic chemicals which readily add alkyl groups to various positions on nucleic acids. A common example is:

TLS Online TPP Program

#Question id: 10414

#SCPH05 I Biotechnology

Plants have evolved with multiple photoreceptors, Phytochrome is one of them. Which of the following statements is incorrect about these photoreceptors?

TLS Online TPP Program

#Question id: 1044

#SCPH06 I Botany

Are all fungi detrimental (bad) for other organisms?

TLS Online TPP Program

#Question id: 24444

#SCPH01 Biochemistry

What is the His bundle electrogram (HBE)?