What type of reaction is shown below?  Amino acid + α-Ketoglutarate⇋ α-Keto acid + Glutamate

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#Question id: 13038

#SCPH06 I Botany

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 13065

#SCPH06 I Botany

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 13024

#SCPH01 Biochemistry

Inset and the vector can be ligated if they are digested by a pair of –

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#Question id: 8689

#SCPH05 I Biotechnology

Which of the following statements best describes the rationale for applying the principle of maximum parsimony in constructing phylogenetic trees?

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#Question id: 935

#SCPH01 Biochemistry

Microorganisms capable of fixing nitrogen into a form usable by animals obtain the energy for this process from ________.