What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

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#Question id: 13081

#SCPH05 I Biotechnology

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 13081

#SCPH06 I Botany

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 13081

#SCPH28 | Zoology

Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 13082

#SCPH01 Biochemistry

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Question id: 13082

#SCPH05 I Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Question id: 13082

#SCPH06 I Botany

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.