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#Question id: 15649
#SCPH05 I Biotechnology
A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g DCW)−1 h−1 is being grown aerobically in a fed-batch reactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s−1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg L−1). The maximum density to which the cells can be grown in the fed-batch process, without the growth being limited by oxygen transfer, is approximately
TLS Online TPP Program
#Question id: 15650
#SCPH05 I Biotechnology
For scaling up of a bioreactor, the following parameter is assumed to be constant
TLS Online TPP Program
#Question id: 15651
#SCPH05 I Biotechnology
Power number, also called Newton’s number, is defined as a dimensionless parameter relating to:
TLS Online TPP Program
#Question id: 15652
#SCPH01 Biochemistry
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation in terms of its basic genetic properties,
TLS Online TPP Program
#Question id: 15653
#SCPH01 Biochemistry
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation on the basis of genetic properties, make a proposal for the type of regulatory functions affected by the MelA– mutation?
TLS Online TPP Program
#Question id: 15653
#SCPH06 I Botany
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation on the basis of genetic properties, make a proposal for the type of regulatory functions affected by the MelA– mutation?