TLS Online TPP Program

#Question id: 568


Which of the following statements about allosteric control of enzymatic activity is false?

#SCPH05 I Biotechnology
  1. Allosteric effectors give rise to sigmoidal V0 vs. [S] kinetic plots.

  2. Allosteric proteins are generally composed of several subunits.

  3. An effector may either inhibit or activate an enzyme.

  4. Heterotropic allosteric effectors compete with substrate for binding sites.

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TLS Online TPP Program

#Question id: 10286

#SCPH05 I Biotechnology

The enzyme aldolase, catalyzes a reversible aldol condensation, Fructose 1,6-bisphosphate is cleaved to yield two different triose phosphates, glyceraldehyde 3-phosphate, an aldose, and dihydroxyacetone phosphate, a ketose. There are two classes of aldolases such as;

A) Class I aldolases

B) Class II aldolases

i) Found in animals and plants

ii) Found in in fungi and bacteria

iii) formation of protonated Schiff base on enzyme and its active site contain lysine residue

iv) do not form the Schiff base intermediate and a zinc ion at the active site is coordinated with the carbonyl oxygen at C-2

In which of the following option is the given enzyme with its correct statements?

TLS Online TPP Program

#Question id: 10287

#I Life Science/ Life Sciences Group – I-V

Glyceraldehyde 3-phosphate dehydrogenase avtive site contain

TLS Online TPP Program

#Question id: 10287

#SCPH05 I Biotechnology

Glyceraldehyde 3-phosphate dehydrogenase avtive site contain

TLS Online TPP Program

#Question id: 10288

#I Life Science/ Life Sciences Group – I-V

 If all the ATP are radiolabelled in the cytosol, then predict about radioactivity of each compound.

 a) All enzyme  of glycolysis will be radiolabelled except 2-phophoglycerate and phosphenol pyruvate

 b) 2-phophoglycerate and phosphenol pyruvate are radiolabelled only the next cycle of glycolysis after cytosolic ATP radiolabelling

 c) 1,3 bisphosphoglycerate will be radiolabelled at 1 and 3 both position

 d) 2-phosphoglycerate are not radiolabelled because its enzyme phosphoglycerate mutase active site contain histidine residue exchange phosphate group from 3-phosphoglycerate

 which prediction about radiolabelling is incorrect?

TLS Online TPP Program

#Question id: 10288

#SCPH05 I Biotechnology

 If all the ATP are radiolabelled in the cytosol, then predict about radioactivity of each compound.

 a) All enzyme  of glycolysis will be radiolabelled except 2-phophoglycerate and phosphenol pyruvate

 b) 2-phophoglycerate and phosphenol pyruvate are radiolabelled only the next cycle of glycolysis after cytosolic ATP radiolabelling

 c) 1,3 bisphosphoglycerate will be radiolabelled at 1 and 3 both position

 d) 2-phosphoglycerate are not radiolabelled because its enzyme phosphoglycerate mutase active site contain histidine residue exchange phosphate group from 3-phosphoglycerate

 which prediction about radiolabelling is incorrect?

TLS Online TPP Program

#Question id: 10289

#I Life Science/ Life Sciences Group – I-V

If C6 of glucose is radiolabelled, which carbon of Dihydroxyacetone phosphate and glyceraldehyde 3-phosphate  is radiolabelled?