In a certain enzyme-catalyzed reaction the following steps occur:

1. A phosphate group on substrate A is transferred to a side chain of an active site residue of the enzyme.

2. The dephosphorylated form of substrate A dissociates from the enzyme.

3. Substrate B enters the active site and is phosphorylated with simultaneous regeneration of the enzyme in its original form.

What kind of kinetic mechanism is described?

TLS Online TPP Program

#Question id: 23163

#SCPH06 I Botany

Out of the given below alexa dyes which one will emit in red light range?

TLS Online TPP Program

#Question id: 23164

#SCPH06 I Botany

Out of the given below alexa dyes which one will emit in Blue light range?

TLS Online TPP Program

#Question id: 23165

#SCPH06 I Botany

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

TLS Online TPP Program

#Question id: 23166

#SCPH06 I Botany

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23167

#SCPH06 I Botany

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

TLS Online TPP Program

#Question id: 23168

#SCPH06 I Botany

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?