TLS Online TPP Program
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TLS Online TPP Program
#Question id: 13088
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 1’s plasmid is;
TLS Online TPP Program
#Question id: 8944
#SCPH28 | Zoology
Which two groups are most clearly represented in the Ediacaran fauna?
TLS Online TPP Program
#Question id: 5699
#SCPH28 | Zoology
A chromosome aberration leads to no change in amount of gene in a genetic map however, appearance of loop in heterozygous condition. This is due to
TLS Online TPP Program
#Question id: 307
#I Life Science/ Life Sciences Group – I-V
An ionic compound A+ B– is most likely to be formed when________
TLS Online TPP Program
#Question id: 555
#I Life Science/ Life Sciences Group – I-V
The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by To determine Km from a double-reciprocal plot, you would: