Red and far-red lights are perceived by plants through various photoreceptors including phytochromes. The red/far-red reversibility of seed germination was due to the-

a) photoreversibility of phytochrome

b) presence of red and far-red light at different fluence rate

c) Conversion of Pr to Pfr form through the effect of red light

d) equal proportion of red and far red light at same fluence rate

Which of the following combinations is correct?

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#Question id: 19708

#SCPH01 Biochemistry

In complete heart block

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#Question id: 5573

#SCPH01 Biochemistry

Animals that have external fertilization are most likely to reproduce in which of the following areas?

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#Question id: 1572

#SCPH05 I Biotechnology

function of IL-10

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#Question id: 309

#SCPH28 | Zoology

Predict the correct order among the following________

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#Question id: 13093

#SCPH06 I Botany

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.