TLS Online TPP Program

#Question id: 14119


Which of the following is incorrect regarding sequence homology?

#SCPH05 I Biotechnology
  1. It is an important concept in sequence analysis
  2. When two sequences are descended from a common evolutionary origin, they are said to share homology
  3. Two sequences can homologous relationship even if have do not have common origin
  4. When two sequences are descended from a common evolutionary origin, they are said to have a homologous relationship
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TLS Online TPP Program

#Question id: 14362

#SCPH05 I Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 

TLS Online TPP Program

#Question id: 14363

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps 

TLS Online TPP Program

#Question id: 14364

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate % increase in activity obtained over the two step  

TLS Online TPP Program

#Question id: 14365

#SCPH05 I Biotechnology

0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 iimol substrate/10 sec. Calculate turnover number (min-1) 

TLS Online TPP Program

#Question id: 14366

#SCPH05 I Biotechnology

0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 mirormol substrate/10 sec. Calculate molecular activity (min^-1), catalytic centre activity (min^-1). - 

TLS Online TPP Program

#Question id: 14367

#SCPH05 I Biotechnology

During an enzyme purification, 0.23 g of protein was dissolved in 5 mL buffer. Suppose 0.5 mL of this solution converted 4.7 mmol of substrate in 30 sec. Calculate specific activity as units/mg protein -