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TLS Online TPP Program

#Question id: 782

Which of the following experimental requirements makes an siRNA approach unsuitable for determining the consequence of silencing a gene of interest in an established cell line?

#SCPH05 I Biotechnology

  1. Expression of the gene of interest is integral to cell viability.

  2. End point RT-PCR and western blots are the only available validation assays.

  3. Lipofectamine is the only available delivery method.

  4. The cell line proliferation rate is relatively slow.

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TLS Online TPP Program

#Question id: 661

#I Life Science/ Life Sciences Group – I-V

Which technique is commonly used to determine the three-dimensional conformation of a protein?
TLS Online TPP Program

#Question id: 12746

#SCPH01 Biochemistry

Choose the methodology which is based on Agglutination reaction.
TLS Online TPP Program

#Question id: 455

#SCPH05 I Biotechnology

Which of the following is correct for a step in a reaction pathway to serve as a control point

A) irreversible  B) endergonic  C) far from equilibrium
TLS Online TPP Program

#Question id: 8924

#I Life Science/ Life Sciences Group – I-V

Fossil evidence indicates that the following events occurred in what sequence, from earliest to most recent?
1. Protostomes invade terrestrial environments.
2. Cambrian explosion occurs.
3. Deuterostomes invade terrestrial environments.
4. Vertebrates become top predators in the seas.
TLS Online TPP Program

#Question id: 562

#SCPH05 I Biotechnology

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme.  This is an example of what kind of inhibition?