#Question id: 13076
#SCPH05 I Biotechnology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
#Question id: 10906
#SCPH05 I Biotechnology
The pressure-flow model explains phloem translocation as a flow of solution (bulk flow) driven by an osmotically generated pressure gradient between source and sink.
a) Phloem translocation, both active and passive mechanisms were considered. All theories, both active and passive, assume an energy requirement in both sources and sinks.
b) In sources, energy is necessary to move photosynthate from producing cells into the sieve elements. This movement of photosynthate is called phloem loading.
c) In sinks, energy is essential for some aspects of movement from sieve elements to sink cells, which store or metabolize the sugar. This movement of photosynthate from sieve elements to sink cells is called phloem unloading.
d) The Active mechanisms of phloem transport further assume that energy is required in the sieve elements of the path between sources and sinks simply to maintain structures.
From the given statements find out the correct for pressure-flow model.
#Question id: 10506
#I Life Science/ Life Sciences Group – I-V
Plant growth promoting rhizobacteria (PGPRs) such as Pseudomonas aeruginosa, that alleviate plant abiotic or biotic stress in soil by releasing of
#Question id: 19374
#SCPH01 Biochemistry
#Question id: 8914
#SCPH06 I Botany