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TLS Online TPP Program
#Question id: 13082
#SCPH28 | Zoology
Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program
#Question id: 13083
#SCPH01 Biochemistry
The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments:
(1) both ends cleaved by PstI (Pst-Pst),
(2) both ends cleaved by MseI (Mse-Mse), and
(3) one end generated by each of the two enzymes (Pst-Mse).
the most frequent fragments in decreasing order,
TLS Online TPP Program
#Question id: 13083
#SCPH05 I Biotechnology
The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments:
(1) both ends cleaved by PstI (Pst-Pst),
(2) both ends cleaved by MseI (Mse-Mse), and
(3) one end generated by each of the two enzymes (Pst-Mse).
the most frequent fragments in decreasing order,
TLS Online TPP Program
#Question id: 13083
#SCPH06 I Botany
The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments:
(1) both ends cleaved by PstI (Pst-Pst),
(2) both ends cleaved by MseI (Mse-Mse), and
(3) one end generated by each of the two enzymes (Pst-Mse).
the most frequent fragments in decreasing order,
TLS Online TPP Program
#Question id: 13083
#SCPH28 | Zoology
The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments:
(1) both ends cleaved by PstI (Pst-Pst),
(2) both ends cleaved by MseI (Mse-Mse), and
(3) one end generated by each of the two enzymes (Pst-Mse).
the most frequent fragments in decreasing order,
TLS Online TPP Program
#Question id: 13084
#SCPH01 Biochemistry
Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.