TLS Online TPP Program

#Question id: 2627


Binding of repressor on to the operator sequences, RNA polymerase binding is analyzed by; Which one is incorrect.

#SCPH05 I Biotechnology
  1. DNA-footprinting

  2. Gel-mobility assays

  3. EMSA

  4. Only A & B

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TLS Online TPP Program

#Question id: 13083

#SCPH28 | Zoology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13084

#SCPH01 Biochemistry

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13084

#SCPH05 I Biotechnology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13084

#SCPH06 I Botany

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13084

#SCPH28 | Zoology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13085

#SCPH01 Biochemistry

The most common microsatellite sequence encountered in human genome is the sequence