What type of single-pass membrane protein would contain a hydrophobic membrane spanning alpha-helix?

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#Question id: 16128

#SCPH01 Biochemistry

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS. 

Next, you decide to evaluate the promoter for the GLN1 gene. To do this you first fuse the promoter region to the LacZ coding sequence and then place this hybrid gene on an appropriate yeast plasmid. You find that cells carrying the hybrid gene express activity under the same conditions that GS is expressed in wild type cells, meaning that the promoter region you have selected contains all of the necessary cis-acting sequences for normal regulation. The figure below shows the effect of different 50 bp deletions in the promoter region on the amount of ß-galactosidase activity expressed by the reporter gene. how would you expect a gln2– gln3– double mutant to behave?

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#Question id: 8942

#SCPH06 I Botany

Which group contains diploblastic organisms?

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#Question id: 4586

#SCPH05 I Biotechnology

 What are adaptations?

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#Question id: 3014

#I Life Science/ Life Sciences Group – I-V

Regarding prokaryotic reproduction, which statement is correct?

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#Question id: 59

#SCPH05 I Biotechnology

Nitrogen (N) normally forms three covalent bonds with a valence of five. However, ammonium has four covalent bonds, each to a different hydrogen (H) atom (H has a valence of one). What do you predict to be the charge on ammonium?