The smallest organism is probably determined by

TLS Online TPP Program

#Question id: 3893

#SCPH01 Biochemistry

Protein structural motifs often have general functions in common.  Which one of the following motifs is known to be involved in protein dimer formation, but not in direct protein-DNA interactions?

TLS Online TPP Program

#Question id: 27709

#Research Methodology

Social Science try to explain …………. Between human activities and natural laws governing them

TLS Online TPP Program

#Question id: 2287

#SCPH01 Biochemistry

Which characteristic do eukaryotic and prokaryotic flagella have in common?

TLS Online TPP Program

#Question id: 3640

#SCPH28 | Zoology

Which of the following is true for the mechanism of excision repair?

TLS Online TPP Program

#Question id: 13092

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?