TLS Online TPP Program

#Question id: 15607


In gene transformation over high expression can be achieved by expressing the gene 
1. Inducible promoter
2. Constitutive  promoter
3. Terminator sequence
4. Increasing copy no. of gene
Which of the following are correct

#SCPH05 I Biotechnology
  1. 1&2 
  2. 2&4 
  3. 2&3 
  4. 1,2,3&4
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TLS Online TPP Program

#Question id: 14165

#SCPH05 I Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                    

Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?

TLS Online TPP Program

#Question id: 14166

#SCPH05 I Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

TLS Online TPP Program

#Question id: 14167

#SCPH05 I Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?


TLS Online TPP Program

#Question id: 14168

#SCPH05 I Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

What is the concentration of solids in the final mixture?

TLS Online TPP Program

#Question id: 14169

#SCPH05 I Biotechnology

Unsweetened condensed milk is a shelf-stable canned milk product. If 100kg of fresh milk, containing 87% water and the rest milk solids, is condensed using an evaporator to remove 60% of the water from the fresh milk, calculate:

 The mass of water evaporated


TLS Online TPP Program

#Question id: 14170

#SCPH05 I Biotechnology

Unsweetened condensed milk is a shelf-stable canned milk product. If 100kg of fresh milk, containing 87% water and the rest milk solids, is condensed using an evaporator to remove 60% of the water from the fresh milk, calculate:

The final mass and composition of condensed milk produced