TLS Online TPP Program
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TLS Online TPP Program
#Question id: 23168
#SCPH06 I Botany
What are the advantages of using secondary antibodies in indirect immunofluorescence?
1. It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program
#Question id: 23169
#SCPH06 I Botany
Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled
TLS Online TPP Program
#Question id: 23170
#SCPH06 I Botany
Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program
#Question id: 23171
#SCPH06 I Botany
Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize
TLS Online TPP Program
#Question id: 23172
#SCPH06 I Botany
Green fluorescent protein do contains a tripeptide sequence i.e. ser-tyr-gly but it only fluoresce when
TLS Online TPP Program
#Question id: 23173
#SCPH06 I Botany
There are various drawbacks still in fluorescence microscopy such as
1. Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?