During a batch culture experiment the following data was obtained 

What will be the product yield (g) per gram of substrates?

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#Question id: 92

#SCPH01 Biochemistry

Monosaccharide derivatives in which an amino group replaces one of the hydroxyl groups may have important roles in

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#Question id: 13091

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

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#Question id: 14142

#I Life Science/ Life Sciences Group – I-V

Which of the following program is not an example of dot plot method?

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#Question id: 5225

#SCPH06 I Botany

Homeotic genes contain a homeobox sequence that is highly conserved among very diverse species. The homeobox is the code for that domain of a protein that binds to DNA in a regulatory developmental process. Which of the following would you then expect?

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#Question id: 13044

#SCPH28 | Zoology

How many different types of dNTPs are utilized in a reaction mixture when carrying out sanger’s DNA sequencing ?