Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

#Question id: 9186

#SCPH06 I Botany

A pathogenic fungus invades a plant. What does the infected plant produce in response to the attack?

#Question id: 2947

#SCPH01 Biochemistry

You perform an experiment where you prevent Cdc20 from binding to APC  in a cell line that usually completes the cell cycle in 60 minutes. Your experiment prevents Cdc20 from binding to APC for 120 minutes, and then you fix and stain the DNA, securin, separase and cohesin proteins. Which of the following would NOT be found in the observed cell?

#Question id: 11086

#I Life Science/ Life Sciences Group – I-V

A 17-year-old female was bicycling without a helmet when she fell and hit her head. In the emergency room, she was not conscious and was receiving ventilator assistance. Her blood gases follow:

PaO2 = 52 mm Hg,

PaCO2 = 75 mm Hg, pH = 7.15, and

HCO3− = 31 mM

The majority of the CO2 was being transported as

#Question id: 3673

#SCPH01 Biochemistry

Which is a characteristic of E. coli DNA polymerases?

#Question id: 15699

#I Life Science/ Life Sciences Group – I-V