A two-stage chemostat system is used for production of secondary metabolite. The volume of each reactor is 0.5 m³; the flow rate of feed is 50 l h^{- 1}. Mycelial growth occurs in the first reactor; the second reactor is used for product synthesis. The concentration of substrate in the feed is 10 gl^-1. Kinetic and yield parameters for the organism are:

                                                        

Assume that product synthesis is negligible in the first reactor and growth is negligible in the second reactor. What is the final concentration of product?

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#Question id: 13085

#SCPH28 | Zoology

The most common microsatellite sequence encountered in human genome is the sequence 

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#Question id: 13086

#SCPH01 Biochemistry

Which group of VNTRs are tandem are frequently distributed in all eukaryotic genomes (except yeast) examined so far. They show a large, stable 'polymorphism due to variation in the number of repeat units and are almost 'ideal as molecular markers for genome mapping.

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#Question id: 13086

#SCPH05 I Biotechnology

Which group of VNTRs are tandem are frequently distributed in all eukaryotic genomes (except yeast) examined so far. They show a large, stable 'polymorphism due to variation in the number of repeat units and are almost 'ideal as molecular markers for genome mapping.

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#Question id: 13086

#SCPH06 I Botany

Which group of VNTRs are tandem are frequently distributed in all eukaryotic genomes (except yeast) examined so far. They show a large, stable 'polymorphism due to variation in the number of repeat units and are almost 'ideal as molecular markers for genome mapping.

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#Question id: 13086

#SCPH28 | Zoology

Which group of VNTRs are tandem are frequently distributed in all eukaryotic genomes (except yeast) examined so far. They show a large, stable 'polymorphism due to variation in the number of repeat units and are almost 'ideal as molecular markers for genome mapping.

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#Question id: 13087

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?