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TLS Online TPP Program

#Question id: 13097

You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
 
If you take any bacterial gene sequence, before you begin doing any sequence analysis on it, there are six potential open reading frames. Why are there six?

#SCPH05 I Biotechnology
  1. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a TGT, a GTT, or a TTC.
  2. There are 6 reading frames on each of two strands because the strand look like the mRNA, then the reading frame could start with a CCC, a CCG, a CGT, a GTA, TAC or a ACG.
  3. There are 3 reading frame only on the top of the strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT.
  4. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a GGG, a GGC, or a GCA.
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TLS Online TPP Program

#Question id: 16137

#SCPH06 I Botany

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)
TLS Online TPP Program

#Question id: 2941

#SCPH05 I Biotechnology

Centromeric Rec8 becomes cleaved during:
TLS Online TPP Program

#Question id: 4947

#SCPH28 | Zoology

The first genes on Earth were probably
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#Question id: 4582

#SCPH05 I Biotechnology

 Fossils found in strata reveal that ________.
TLS Online TPP Program

#Question id: 10956

#SCPH28 | Zoology

The coagulation pathway that begins with tissue thromboplastin is