To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 4334

#SCPH06 I Botany

Which of the following is not true of RNA processing?

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#Question id: 4748

#SCPH28 | Zoology

Eye color in Drosophila present on X chromosome, Red eye color is dominant over white eye color. There is non disjunction in Meiosis I in female and normal meiosis in male. Female is white eye color was cross with red eye male. What will be expected phenotype in their progeny?

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#Question id: 18616

#SCPH01 Biochemistry

In a PCR higher annealing temperature along with7-dcaza guanosine triphosphate (7-deaza OTP) used in place of GTP this indicate

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#Question id: 1651

#I Life Science/ Life Sciences Group – I-V

Ig idiotypes are found:

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#Question id: 16136

#SCPH06 I Botany

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?