To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 5324

#SCPH06 I Botany

In eusocial insects, males develop from unfertilized eggs while females develop from fertilized eggs. The ultimate consequence of this difference is that

#Question id: 9158

#SCPH28 | Zoology

Coral reefs occur on the southeast coast of the United States but not at similar latitudes on the southwest coast. Differences in which of the following most likely account for this?

#Question id: 12936

#SCPH28 | Zoology

#Question id: 11272

#SCPH28 | Zoology

Measurement of phytoplankton production, take sample of phytoplankton suspended in three bottles in lake. One bottle as control assumed as initial, second bottle (D) was kept in dark while third bottle (L) was kept in light. After some time productivity measure fallow

A- Difference in dissolved oxygen between light bottle and dark bottle yield NET primary productivity

B- Difference in dissolved oxygen between light bottle and initial bottle yield NET primary productivity

C- Difference in dissolved oxygen between light bottle and initial bottle yield Grass primary productivity

D- Difference in dissolved oxygen between light bottle and dark bottle yield total oxygen produced by photosynthesis

Which above combination of statement are correct?

#Question id: 359

#SCPH28 | Zoology

What is the concentration of OH− in a solution with an H+ concentration of 1.3 × 10^−4 M?