To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 7316

#SCPH06 I Botany

Programmed cell death, or apoptosis, is an essential part of animal development. In the nematode, with its invariant cell lineage, particular cell lineages or particular cells within specific lineages are destined to end in programmed cell death, and it was this feature that allowed the identification of the process and the analysis of its genetic control.

Which of the following combination of genes are correct?

#Question id: 7316

#SCPH28 | Zoology

Programmed cell death, or apoptosis, is an essential part of animal development. In the nematode, with its invariant cell lineage, particular cell lineages or particular cells within specific lineages are destined to end in programmed cell death, and it was this feature that allowed the identification of the process and the analysis of its genetic control.

Which of the following combination of genes are correct?

#Question id: 7696

#SCPH06 I Botany

The following question refers to the following phylogenetic trees. Which tree depicts the microsporidians as a sister group of the ascomycetes?

#Question id: 7696

#SCPH28 | Zoology

The following question refers to the following phylogenetic trees. Which tree depicts the microsporidians as a sister group of the ascomycetes?

#Question id: 7697

#SCPH06 I Botany

The following question refers to the following phylogenetic trees. Which tree depicts the closest relationship between zygomycetes and chytrids?

#Question id: 7697

#SCPH28 | Zoology

The following question refers to the following phylogenetic trees. Which tree depicts the closest relationship between zygomycetes and chytrids?