Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

TLS Online TPP Program

#Question id: 3631

#SCPH12 I Genetics

Six different mutations 1 to 6, from the following results of functional allelism tests whereby the mutants were crossed with each other, determine location of mutant gene.

TLS Online TPP Program

#Question id: 11067

#I Life Science/ Life Sciences Group – I-V

A preterm infant has a surfactant deficiency. Without surfactant, many of the alveoli collapse at the end of each expiration, which in turn leads to pulmonary failure. Which of the following sets of changes are present in the preterm infant, compared to a normal infant?

TLS Online TPP Program

#Question id: 2705

#SCPH05 I Biotechnology

With respect to end terminal modification of the histone tail which of the following options is true?

TLS Online TPP Program

#Question id: 8862

#SCPH06 I Botany

The members of which class of the phylum Cnidaria occur only as polyps?

TLS Online TPP Program

#Question id: 544

#SCPH28 | Zoology

One of the enzymes involved in glycolysis, aldolase, requires Zn2+ for catalysis. Under conditions of zinc deficiency, when the enzyme may lack zinc, it would be referred to as the: