Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 11538

#SCPH28 | Zoology

Prostaglandins are local regulators whose chemical structure is derived from ________.

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#Question id: 4799

#SCPH06 I Botany

You set up a mapping cross involving your favorite gene (YFG) in Neurospora and determine the following octad types: 4:4 arrangement 83; 2:4:2 arrangement 7; 2:2:2:2 arrangement 10. What is the distance between your gene and the centromere?

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#Question id: 546

#SCPH01 Biochemistry

Enzymes are potent catalysts because they:

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#Question id: 79

#SCPH05 I Biotechnology

The structure of D-arabinose is shown below. How many stereoisomers are possible for this molecule?

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#Question id: 1563

#SCPH06 I Botany

A nonfunctional CD4 protein on a helper T cell would result in the helper T cell being unable to ________.