Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 380

#SCPH05 I Biotechnology

The Henderson-Hasselbalch equation:

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#Question id: 5538

#SCPH28 | Zoology

The first truly vertebrate phase of development is manifested by the formation of the

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#Question id: 5027

#SCPH28 | Zoology

Fossil evidence indicates that the following events occurred in what sequence, from earliest to most recent?

1. Protostomes invade terrestrial environments.

2. Cambrian explosion occurs.

3. Deuterostomes invade terrestrial environments.

4. Vertebrates become top predators in the seas.

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#Question id: 4805

#SCPH12 I Genetics

What is maximum parental frequency for two genes if single cross over within two strands?

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#Question id: 373

#SCPH01 Biochemistry

What is the hydroxyl ion (OH-) concentration of a solution of pH 8?