Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 27757

#Research Methodology

A ________ refers to some difficulty that a researcher experiences in either a theoretical or practical situation

TLS Online TPP Program

#Question id: 27758

#Research Methodology

_______ as a testable statement of a potential relationship between two or more variables

TLS Online TPP Program

#Question id: 27759

#Research Methodology

Research design is a _________for conducting the marketing research project.

TLS Online TPP Program

#Question id: 27760

#Research Methodology

______ is a hypothetical statements denying what are explicitly indicated in working hypotheses. 

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#Question id: 27761

#Research Methodology

A Blue print of Research work is known as 

TLS Online TPP Program

#Question id: 27762

#Research Methodology

Research design is a blue print, outline and a __