Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 16096

#SCPH28 | Zoology

heat stroke is characteristically associated with

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#Question id: 14108

#SCPH05 I Biotechnology

The laboratory work using computers and associated with web-based analysis generally online is referred to as __________.

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#Question id: 4730

#SCPH06 I Botany

Cross is made between a pure breeding plant having red coloured male flowers with a pure breeding plant having white colored female flowers. All of these offspring are white colored flower. Upon selfing of these F1 red flower plant produced both red and white flower. Which of the following is conclusion of these cross?

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#Question id: 2955

#SCPH28 | Zoology

Cyclin is to MPF as ............ is to cyclin-dependent kinases.

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#Question id: 18000

#SCPH05 I Biotechnology

Two-dimensional polyacrylamide gel electrophoresis separates the proteins on the basis of