Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 838

#SCPH06 I Botany

Which of the following correct predict the transition state?

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#Question id: 7007

#SCPH28 | Zoology

The change in the state of specification of imaginal disc of Drosophila to that of a different disc type is known as:

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#Question id: 5025

#SCPH28 | Zoology

Which of these genetic processes may be most helpful in accounting for the Cambrian explosion?

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#Question id: 4585

#SCPH06 I Botany

The cow Bos primigenius (which is bred for meat and milk) has a smaller brain and larger eyes than closely related wild species of ungulates. These traits most likely arose by ________.

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#Question id: 10509

#SCPH05 I Biotechnology

A different type of mechanical obstacle to herbivory is provided by mineral crystals that are present in many plant species, such as silica crystals known as