Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Question id: 5426

#SCPH06 I Botany

Which of the following is incorrect regard polygenic inheritance?

#Question id: 5426

#SCPH28 | Zoology

Which of the following is incorrect regard polygenic inheritance?

#Question id: 5427

#SCPH06 I Botany

In a plant, height is control by three quantitative trait loci.  Each dominant additive allele is contributing 3 cm height in plant. Maximum expected plant height is 20 cm. What is minimum expected height? 

#Question id: 5427

#SCPH28 | Zoology

In a plant, height is control by three quantitative trait loci.  Each dominant additive allele is contributing 3 cm height in plant. Maximum expected plant height is 20 cm. What is minimum expected height? 

#Question id: 5428

#SCPH06 I Botany

If trait are governed by polygenic. If plant genotype AABB  ( 8 cm height ) were cross with aabb ( 2 cm height ) produced F1 and allowed to selfing . which of the following phenotypic height are not observe in F2 plant if all gamete participate in fertilization ?

#Question id: 5428

#SCPH28 | Zoology

If trait are governed by polygenic. If plant genotype AABB  ( 8 cm height ) were cross with aabb ( 2 cm height ) produced F1 and allowed to selfing . which of the following phenotypic height are not observe in F2 plant if all gamete participate in fertilization ?