Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 4610

#SCPH28 | Zoology

It has been observed that organisms on islands are different from, but closely related to, similar forms found on the nearest continent. This is taken as evidence that ________.

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#Question id: 4611

#SCPH28 | Zoology

Given what we know about evolutionary biology, we expect to find the largest number of endemic species in which of the following geological features, which have existed for at least a few million years?

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#Question id: 4718

#SCPH06 I Botany

All the following statements about destruction of cyclins are true, except:

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#Question id: 4719

#SCPH01 Biochemistry

When a trait exhibits complete co-dominance rather than complete dominance, a cross between heterozygote results deviation of following;

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#Question id: 4719

#SCPH06 I Botany

When a trait exhibits complete co-dominance rather than complete dominance, a cross between heterozygote results deviation of following;

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#Question id: 4719

#SCPH12 I Genetics

When a trait exhibits complete co-dominance rather than complete dominance, a cross between heterozygote results deviation of following;