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#Question id: 13031


Use of alkaline solution in southern blotting helps in :

#SCPH05 I Biotechnology
  1. Transfer of the blot to nitrocellulose membrane
  2. Depurination of DNA fragments and hence breaking them into smaller fragments
  3. Denaturation of the DNA fragments helping in probe hybridization
  4. Binding of negatively charged DNA to the membrane

Answer:- Option(s): 3


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