For optimum callus growth, the given some following vitamins are required, except;

#Question id: 18590

#SCPH28 | Zoology

#Question id: 643

#SCPH05 I Biotechnology

The hydrolysis of lactose is catalysed by b-galactosidase as given below

Which of the following could be correct mechanism for this hydrolysis reaction?

A. Nucleophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.

B. Electrophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.

C. Nucleophilic attack by the carboxylate group of Lys/Arg/His or bivalent cation on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.

#Question id: 10507

#I Life Science/ Life Sciences Group – I-V

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?

#Question id: 27906

#Research Methodology

#Question id: 544

#I Life Science/ Life Sciences Group – I-V

One of the enzymes involved in glycolysis, aldolase, requires Zn2+ for catalysis. Under conditions of zinc deficiency, when the enzyme may lack zinc, it would be referred to as the: