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#Question id: 12904
#SCPH01 Biochemistry
Match the following:
1. Southern Blotting i. Melting temperature of DNA strand
2. DNA microchips ii.oligonucleotide matching to DNA sample
3. ASO-PCR iii.DNA hybridization to oligo arrays
4. DGGE iv. labeled probe hybridization to DNA
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#Question id: 15154
#SCPH01 Biochemistry
In this problem we will explore some of the many ways that mutations in two different genes can interact to produce different Mendelian ratios. Consider a hypothetical insect species that has red eyes. Imagine mutations in two different unlinked genes that can, in certain combinations, block the formation of red eye pigment yielding mutants with white eyes. In principle, there are two different possible arrangements for two biochemical steps responsible for the formation of red eye pigment. The two genes might act in series such that a mutation in either gene would block the formation of red pigment. Alternatively, the two genes could act in parallel such that mutations in both genes would be required to block the formation of red pigment.
Further complexity arises from the possibility that mutations in either gene that lead to a block in enzymatic activity could be either dominant or recessive. If the crosses between a wild type insect with red eyes and a true breeding white eyed strain with mutations in both genes. Such considerations yield the Pathways in parallel with a recessive mutation in one gene and a dominant mutation in the other, determine the phenotype of the F1 progeny and the expected phenotypic ratio of red to white eyed insects in the F2.
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#Question id: 4901
#SCPH06 I Botany
Assume that the alleles A (dominant) and a (recessive) show a maternal effect mode of inheritance. In a cross between an Aa female and an aa male, what would be the phenotypes of the progeny?
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#Question id: 4344
#SCPH05 I Biotechnology
How does the transcription of structural genes in an inducible operon occur?
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#Question id: 1672
#SCPH01 Biochemistry
Using only random VDJ recombination, from 40 V, 23 D, and 6 J gene segments, what are the number of possible variable regions of an antigen receptor molecule?