Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

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#Question id: 15182

#SCPH06 I Botany

Which of the following types of effect are responsible for lowering the efficiency of electrophoresis?

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#Question id: 15182

#SCPH28 | Zoology

Which of the following types of effect are responsible for lowering the efficiency of electrophoresis?

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#Question id: 15183

#SCPH01 Biochemistry

When in a gel more Joule heat is produced than can be dissipated, the temperature in the middle of the gel is higher than at the lateral edges. This causes higher mobility of the ions in the center, resulting in an uneven distribution of the zones. The zones bend up at the two lateral sides, causing

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#Question id: 15183

#SCPH05 I Biotechnology

When in a gel more Joule heat is produced than can be dissipated, the temperature in the middle of the gel is higher than at the lateral edges. This causes higher mobility of the ions in the center, resulting in an uneven distribution of the zones. The zones bend up at the two lateral sides, causing

TLS Online TPP Program

#Question id: 15183

#SCPH06 I Botany

When in a gel more Joule heat is produced than can be dissipated, the temperature in the middle of the gel is higher than at the lateral edges. This causes higher mobility of the ions in the center, resulting in an uneven distribution of the zones. The zones bend up at the two lateral sides, causing

TLS Online TPP Program

#Question id: 15183

#SCPH28 | Zoology

When in a gel more Joule heat is produced than can be dissipated, the temperature in the middle of the gel is higher than at the lateral edges. This causes higher mobility of the ions in the center, resulting in an uneven distribution of the zones. The zones bend up at the two lateral sides, causing