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#Question id: 4144
#SCPH28 | Zoology
The Wobble Hypothesis suggests that:
TLS Online TPP Program
#Question id: 5260
#SCPH28 | Zoology
Regarding prokaryotic reproduction, which statement is correct?
TLS Online TPP Program
#Question id: 8840
#SCPH06 I Botany
There are at least a dozen known species in the extinct genus Lystrosaurus. If each species was suited to a quite different environment, then this relatively large number of species is likely due to
TLS Online TPP Program
#Question id: 13092
#SCPH06 I Botany
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?
TLS Online TPP Program
#Question id: 15617
#SCPH01 Biochemistry
Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;