Some statements regarding to hippo pathway.

A. The Hippo signal transduction pathway have a dedicated ligand or receptor

B. Hippo stands for one of several important kinases that are critical for organ size control

C. It was first identified in Drosophila, where its loss resulted in a “hippopotamus”-shaped phenotype due to excessive growth

D. Gain of Hippo causes cells to divide significantly faster while slowing apoptosis

E. overexpression of its main transcriptional effector, Yorkie causes cells to divide significantly faster while slowing apoptosis

Which of the following combination are incorrect?

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#Question id: 2996

#I Life Science/ Life Sciences Group – I-V

Cell death approximately equal to cell division is found in

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#Question id: 13089

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

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#Question id: 2927

#SCPH28 | Zoology

Site-directed mutagenesis of Tyr-15 to Phe in cdc2:

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#Question id: 18644

#SCPH01 Biochemistry

A laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge.

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#Question id: 4802

#SCPH28 | Zoology

Why is tetrad analysis useful for studying genetics?