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TLS Online TPP Program
#Question id: 14741
#SCPH05 I Biotechnology
Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What operating speed is required to recover 90% of cells if the flow rate in the centrifuge is maintained at 601/min?
TLS Online TPP Program
#Question id: 14742
#SCPH05 I Biotechnology
Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-4m3, Wr = 3x10-3m3 /kg dry sephacryl, ps =1.25 x 103 kg/m3 . The partition coefficients are kpA =0.38 and kpB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone?
TLS Online TPP Program
#Question id: 14743
#SCPH05 I Biotechnology
for the fermenter with the given data.
Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P
TLS Online TPP Program
#Question id: 14744
#SCPH05 I Biotechnology
For production of an enzyme used for synthesis of a sun protection lotion, the required kinetic data and constants are:
vmax = 2.3 mmol.m-3 s-1 Km = 8.9 mmol.-1 So = 12 mmol1-
Find the reaction time for 95% conversion of raw material
TLS Online TPP Program
#Question id: 14745
#SCPH05 I Biotechnology
heat exchanger, one transfer unit means
TLS Online TPP Program
#Question id: 14746
#SCPH05 I Biotechnology
During cultivation of microorganism in a fermenter, various parameters are controlled by appropriate mechanism, match the following