Consider the accompanying pedigree of a rare autosomal recessive disease, PKU. Male with PKU marry with normal female whose father is PKU. What is probability of child affected with PKU?

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#Question id: 13091

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

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#Question id: 3637

#SCPH01 Biochemistry

Parents that are homozygous for different mutations a, b and c are crossed, producing offspring that are heterozygous as following

aa X bb        =  ab  (X)          

 bb X cc         =  bc  (Y)

aa X cc          =   ac  (Z)

If a and b belong to same locus but b and c belong to different locus then phenotype of heterozygote

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#Question id: 1704

#SCPH05 I Biotechnology

The single best unique description of the interaction of the αβ T-cell receptor with peptide-MHC is that it:

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#Question id: 7300

#SCPH06 I Botany

Following genes regulate flowering, column I represents genes, column II represents transcription factor family and column III represents functions.

Column I	Column II	Column III
A. FLOWERING D (FD)	i. bZIP	a. Activates flowering downstream of florigen
B. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)	ii. MADS	b. Receptor for florigen
C. FLOWERING LOCUS C		c. Class E homeotic genes
D. SEPALLATA (SEP)		d. Floral repressor

 Which of the following is correct?

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#Question id: 52

#SCPH05 I Biotechnology

Choose the incorrect option regarding Isomerism.